H2S2-triggered off-on fluorescent indicator with endoplasmic reticulum targeting for imaging in cells and zebrafishes
| Autor: | Jinbao Tang, Bochao Chen, Jian Zhang, Hui Zhou, Jie Zhang, Jin Zhou, Weifen Zhang, Lin Sun, Jianfei Kan |
|---|---|
| Rok vydání: | 2019 |
| Předmět: |
Cell signaling
biology Chemistry Endoplasmic reticulum Metals and Alloys Endogeny 02 engineering and technology 010402 general chemistry 021001 nanoscience & nanotechnology Condensed Matter Physics biology.organism_classification 01 natural sciences 0104 chemical sciences Surfaces Coatings and Films Electronic Optical and Magnetic Materials Cell biology In vivo Materials Chemistry Electrical and Electronic Engineering 0210 nano-technology Cytotoxicity Instrumentation Zebrafish Transcription factor Ion channel |
| Zdroj: | Sensors and Actuators B: Chemical. 278:64-72 |
| ISSN: | 0925-4005 |
| DOI: | 10.1016/j.snb.2018.09.081 |
| Popis: | Reactive sulfur species (RSS) play important physiological and pathological roles in biological redox homeostasis, cell signaling and the regulation of metabolism. Recent studies indicate that hydrogen polysulfides (H2Sn) are the actual signal molecules in animal cells. Although H2Sn have been implied to be answered for transcription factors, ion channels, and mediating tumor suppressors, more of their biological effects such as the effect of a specific hydrogen polysulfide in subcellular organelle are still needed to be elaborated. The detection of a specific hydrogen polysulfide (e.g. H2S2) in living systems is essential for investigating their functions but is quite challenging. Herein, we report an off-on fluorescent probe ER-H2S2 with a favorable photophysical property, high specificity, wide linear range and low cytotoxicity via a facile synthesis for the detection of H2S2 endoplasmic reticulum-selectively in living cells and in zebrafish models in vivo, giving an example of the use of fluorescent probes in real-time imaging exogenous and endogenous H2S2 in subcellular organelle during the course of inflammation and anti-inflammation. |
| Databáze: | OpenAIRE |
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