Thin coatings based on ZnO@C18-usnic acid nanoparticles prepared by MAPLE inhibit the development of Salmonella enterica early biofilm growth
| Autor: | Miruna Silvia Stan, Ana Maria Ene, Anca Dinischiotu, Mariana Carmen Chifiriuc, Alexandru Mihai Grumezescu, Sabrina Constanda, Bogdan Stefan Vasile, Valentina Grumezescu, Veronica Lazar, Ecaterina Andronescu, George Dan Mogoşanu, Tudor-Adrian Bălşeanu, Gabriel Socol, Alina Maria Holban, Laurenţiu Mogoantă |
|---|---|
| Rok vydání: | 2016 |
| Předmět: |
Materials science
General Physics and Astronomy Nanoparticle Nanotechnology 02 engineering and technology 010402 general chemistry 01 natural sciences chemistry.chemical_compound MTT assay Cytotoxicity biology Biofilm Usnic acid Surfaces and Interfaces General Chemistry 021001 nanoscience & nanotechnology Condensed Matter Physics biology.organism_classification 0104 chemical sciences Surfaces Coatings and Films chemistry Salmonella enterica Sodium stearate Formazan 0210 nano-technology Nuclear chemistry |
| Zdroj: | Applied Surface Science. 374:318-325 |
| ISSN: | 0169-4332 |
| DOI: | 10.1016/j.apsusc.2015.12.063 |
| Popis: | The aim of this study was to develop a nanostructured bioactive surface based on zinc oxide, sodium stearate (C18) and usnic acid (UA) exhibiting harmless effects with respect to the human cells, but with a significant antimicrobial effect, limiting the attachment and biofilm formation of food pathogens. ZnO nanoparticles were synthesized by sol–gel method and functionalized with C18 and UA. The coatings were fabricated by matrix assisted pulsed laser evaporation technique (MAPLE) and further characterized by TEM, SEM, SAED, XRD and IRM. The biological characterization of the prepared coatings consisted in cytotoxicity and antimicrobial assays. The cytotoxicity of ZnO@C18 and ZnO@C18-UA films was evaluated with respect to the human skin fibroblasts (CCD 1070SK cell line) by phase contrast microscopy, MTT assay and nitric oxide (NO) release. The covered surfaces exhibited a decreased cell attachment, effect which was more pronounced in the presence of UA as shown by purple formazan staining of adhered cells. The unattached fibroblasts remained viable after 24 h in the culture media as it was revealed by their morphology analysis and NO level which were similar to uncovered slides. The quantitative microbiological assays results have demonstrated that the bioactive coatings have significantly inhibited the adherence and biofilm formation of Salmonella enterica. The obtained results recommend these materials as efficient approaches in developing anti-adherent coatings for various industrial, medical and food processing applications. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full Text from ScienceDirect