Pol B, a Family B DNA Polymerase, in Thermococcus kodakarensis is Important for DNA Repair, but not DNA Replication
| Autor: | Issay Narumi, Tadayuki Imanaka, Shinsuke Fujiwara, Hiroki Higashibata, Yoshizumi Ishino, Takashi Kushida, Sonoko Ishino |
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| Rok vydání: | 2019 |
| Předmět: |
0303 health sciences
biology 030306 microbiology Chemistry DNA polymerase DNA repair DNA replication Soil Science Plant Science General Medicine biology.organism_classification Molecular biology Thermococcus kodakarensis 03 medical and health sciences chemistry.chemical_compound Plasmid biology.protein Thermococcus Ecology Evolution Behavior and Systematics Polymerase DNA 030304 developmental biology |
| Zdroj: | Microbes and Environments. 34:316-326 |
| ISSN: | 1347-4405 1342-6311 |
| DOI: | 10.1264/jsme2.me19075 |
| Popis: | Thermococcus kodakarensis possesses two DNA polymerases, Pol B and Pol D. We generated a T. kodakarensis strain (DPB1) in which polB was completely deleted and a derivative of DPB1 in which polB was overexpressed; neither of the generated strains exhibited any growth delay, indicating that the lack or overexpression of Pol B in T. kodakarensis did not affect cell growth. We also found that DPB1 showed higher sensitivity to four DNA-damaging agents (ultraviolet C irradiation, γ-ray irradiation, methyl methanesulfonate, and mitomycin C) than the parental strain. The sensitivity of DPB1 was restored to the level of the parent strain by the introduction of a plasmid harboring polB, suggesting that the DNA damage-sensitive phenotype of DPB1 was due to the loss of polB. Collectively, these results indicate that Pol B is involved in DNA repair, but not DNA replication, which, in turn, implies that Pol D is the sole replicative DNA polymerase in Thermococcus species. |
| Databáze: | OpenAIRE |
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