| Autor: |
Alexander Ludwig, Christina Birgitte R. Rasmussen, Nicoline Fürstenberg, Maarten Altelaar, Anna Akhmanova, Stine K. Morthorst, Zeinab Anvarian, Søren T. Christensen, Camilla Nielsen, Ilya Grigoriev, Pietro Farinelli, Lotte B. Pedersen, Andrea Serra-Marques |
| Rok vydání: |
2021 |
| Předmět: |
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| Popis: |
The kinesin-3 motor KIF13B functions in endocytosis, vesicle transport, and regulation of ciliary length and signaling. Direct binding of the membrane-associated guanylate kinase (MAGUK) DLG1 to KIF13B’s MAGUK-binding stalk (MBS) domain relieves motor autoinhibition and promotes microtubule plus end-directed cargo transport. Here we characterize Angiomotin isoform 2 (Ap80) as a novel KIF13B interactor that promotes binding of another MAGUK, the polarity protein and Crumbs complex component PALS1, to KIF13B. Live cell imaging analysis indicated that Ap80 is concentrated at the base of primary cilia and recruits PALS1 to this site, but is not itself a cargo of KIF13B. Consistent with a ciliary function for Ap80, its depletion led to elongated primary cilia while its overexpression caused ciliary shortening. Our results suggest that Ap80 may specifically activate KIF13B cargo binding at the base of primary cilia to regulate ciliary length. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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