Whole Transcriptome Sequencing Analysis of Cancer Stem/Progenitor Cells Obtained from Mouse Lung Adenocarcinomas
| Autor: | Wassim Abou-Kheir, Humam Kadara, Ansam Sinjab, Reem Daouk |
|---|---|
| Rok vydání: | 2021 |
| Předmět: |
0303 health sciences
Cancer Tumor initiation Biology medicine.disease medicine.disease_cause In vitro 03 medical and health sciences 0302 clinical medicine 030220 oncology & carcinogenesis Gene expression Cancer research medicine Progenitor cell Stem cell Lung cancer Carcinogenesis 030304 developmental biology |
| Zdroj: | Methods in Molecular Biology ISBN: 9781071612774 |
| DOI: | 10.1007/978-1-0716-1278-1_15 |
| Popis: | Interrogation and characterization of lung cancer stem cells (CSCs) that are implicated in lung oncogenesis is crucial for our understanding of inter- and intra-tumor heterogeneity and the aggressive nature of the disease, including tumor resistance and relapse in response to conventional therapy. Here, we describe an in vitro surrogate model, namely the "sphere-forming assay," for the derivation, enrichment, and propagation of lung stem/progenitor cells with CSC properties, including self-renewal, tumor initiation capacity and propagation, and differentiation into cells of the tumor bulk, from a murine Kras-mutant lung adenocarcinoma cell line. Self-renewing cancer stem/progenitor cells, in the form of 3D in vitro spheres, can be phenotypically interrogated using downstream techniques such as gene expression analysis (e.g., whole transcriptome sequencing and quantitative real time PCR), which is the focus of this chapter. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|