Measuring temporal expression, systematic response, and post-mortem stability to assess potential markers for estimating wound age: an example of Fosl1 in contused skeletal muscle
| Autor: | San-qiang Li, Ta-na Dong, Jun-hong Sun, Qiu-xiang Du, Xi-yan Zhu |
|---|---|
| Rok vydání: | 2017 |
| Předmět: |
Pathology
medicine.medical_specialty Pentobarbital 010401 analytical chemistry Chloral hydrate Lethal dose Skeletal muscle Biology FOSL1 01 natural sciences 0104 chemical sciences Pathology and Forensic Medicine Blot 03 medical and health sciences 0302 clinical medicine Real-time polymerase chain reaction medicine.anatomical_structure Age estimation medicine 030216 legal & forensic medicine medicine.drug |
| Zdroj: | Australian Journal of Forensic Sciences. 51:158-170 |
| ISSN: | 1834-562X 0045-0618 |
| DOI: | 10.1080/00450618.2017.1334824 |
| Popis: | To assess whether Fosl1 is a suitable parameter for wound age estimation, a total of 126 male Sprague–Dawley rats were divided into control (n = 6 per group), contusion (including 4, 8, 12, 16, 20, 24, 28, 32, 36, 40, 44, and 48 h post-severe injury, and 4 or 8 h post-moderate or -mild injury subgroups), and post-mortem (including 6, 12, 18, and 24 h subgroups) groups (n = 6 per subgroup). A contusion was produced in the right limb of the rats using the drop-ball technique (under intraperitoneal chloral hydrate anaesthesia), and the animals were sacrificed using a lethal dose of pentobarbital. The expression of Fosl1 mRNA and protein was determined in the contused and contralateral uninjured muscle and post-mortem specimens of musculi quadriceps femoris using real-time PCR and western blotting, respectively. Compared with the control group, the expression of Fosl1 mRNA was increased in several contused and contralateral uninjured muscles, and decreased in post-mortem groups (P |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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