Utilizing electric field to separate cells with travelling-wave dielectrophoresis force in a droplet
| Autor: | Adel Pourmand, Jino Fathy, Habib Badri Ghavifekr |
|---|---|
| Rok vydání: | 2017 |
| Předmět: |
Materials science
010401 analytical chemistry Nanotechnology 02 engineering and technology Mechanics Dielectrophoresis 021001 nanoscience & nanotechnology Curvature 01 natural sciences 0104 chemical sciences Microelectrode Amplitude Electric field Electrode Traveling wave 0210 nano-technology Separator (electricity) |
| Zdroj: | 2017 Iranian Conference on Electrical Engineering (ICEE). |
| DOI: | 10.1109/iraniancee.2017.7985489 |
| Popis: | This work presents utilizing of electric field to separate viable and nonviable yeast cells in a droplet. The separator consists of two parallel plates with deposited electrodes and a droplet is placed between them. Bottom electrodes are boomerang-shaped and are used for cell separation by travelling-wave dielectrophoresis. The upper electrodes are square-shaped and are utilized for cutting the droplet, after the cell separation. Bottom microelectrodes are excited with 90 degrees' phase shifted electric potentials with amplitude of 4 V p-p and frequency of 70 kHz. In this situation, nonviable yeast cells are not significantly affected by travelling-wave dielectrophoresis while viable ones are focused at the center of the curvature of the electrodes. This is a significant advantage of our proposed in-droplet separator, because ensures the accumulation of viable yeast cells at the rightest corner of the droplet and guarantees that they remain at the right daughter droplet through the droplet cutting process. The design process and simulations are explained. Simulations have been done in a commercially available finite element software to simulate electric field and predict the cell trajectories. Then the results are validated by comparing them with previous works in the literature. |
| Databáze: | OpenAIRE |
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