UV Detection and Avoidance of Protein in Basella alba leaf Mucilage Polysaccharide by differential precipitation
| Autor: | Duraiswamy B, Priyadarshini S, Arvind P, Ramu G, S. P. Dhanabal |
|---|---|
| Rok vydání: | 2021 |
| Předmět: |
0301 basic medicine
chemistry.chemical_classification 030109 nutrition & dietetics Chromatography biology Chemistry Precipitation (chemistry) 04 agricultural and veterinary sciences biology.organism_classification Polysaccharide 040401 food science Basella 03 medical and health sciences 0404 agricultural biotechnology Mucilage - Polysaccharide Mucilage Pharmacology (medical) Uv detection Pharmacology Toxicology and Pharmaceutics (miscellaneous) |
| Zdroj: | Research Journal of Pharmacy and Technology. :3093-3096 |
| ISSN: | 0974-360X 0974-3618 |
| DOI: | 10.52711/0974-360x.2021.00540 |
| Popis: | Objective: In the aim of refining mucilage polysaccharide extracted from the leaf of Basella alba, extraction and differential precipitation of the protein content was studied. This was attempted by pH adjustment by adding Trichloroacetic acid (TCA) and Hydrochloric acid (HCl). Materials and Methods: The presence of residual protein before and after deproteinization in the polysaccharide solution was detected by UV spectrum analysis. The % polysaccharide, % polysaccharide loss, protein concentration and the deproteinization efficiency were studied as comparative indices to evaluate the precipitation experimental conditions using pH adjustment. Results: The result showed that 10% w/v TCA precipitated over 80% of the protein when the pH of the aqueous polysaccharide solution was 3. Discussion: TCA was proved to be superior to hydrochloric acid as evidenced by the highest deproteinization efficiency (83.3%). The polysaccharides of all the extracted solutions obtained were identified with only slight variations in percentage. The Hcl method excelled over the TCA method in obtaining polysaccharides with little lower percentage of polysaccharide loss (13.94 %). Conclusion: The TCA method will offer a room for deproteinization of polysaccharides if optimization is studied. |
| Databáze: | OpenAIRE |
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