Optimizing in vitro factors for improvement of shoot proliferation of ‘Flordaguard’ peach rootstock

Autor:	Simone Ribeiro Lucho, Elizete Beatriz Radmann, Valmor João Bianchi, Cristina Weiser Ritterbusch
Rok vydání:	2021
Předmět:	0106 biological sciences 0301 basic medicine Chemistry food and beverages Plant Science 01 natural sciences In vitro 03 medical and health sciences Horticulture 030104 developmental biology Shoot Rootstock 010606 plant biology & botany Explant culture
Zdroj:	Iheringia, Série Botânica. 76:e2021011-e2021011
ISSN:	2446-8231 0073-4705
Popis:	‘Flordaguard’ has shown great potential as a standard rootstock for peach. The aim of this study was to examine the influence of Murashige & Skoog (MS) medium and modified MS-½N medium (half Nitrogen content), explant type and angle orientation on the culture medium, as well as 6-benzylaminopurine (BAP) concentrations on in vitro shoot proliferation in ‘Flordaguard’ rootstock. Explants cultured in MS-½N medium showed higher averages for number of lateral shoots and elongated shoots. The maximum average length of lateral shoots was obtained using doublephase medium. The 3.0 mg L-1 BAP induced the highest number of lateral shoots per explant (3.38 ±0.57). Whereas the higher number of lateral shoots elongated (1.75 ±0.10 cm) occurred at concentrations until 2.0 mg L-1 BAP. The study corroborated the importance of a 50% reduction in both NH4NO3 and KNO3 content in the cultured medium to improvein vitro propagation of ‘Flordaguard’ peach rootstock.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::7d819a59bec8a0e7ac6f6f11f3bb55c9 https://doi.org/10.21826/2446-82312021v76e2021011 Zobrazit plný text záznamu