Serum IgE Antibodies to Cow's Milk Proteins in Children With Differing Degrees of IgE-Mediated Cow's Milk Allergy: Analysis by Immunoblotting
| Autor: | Robert H. Schwartz, Stephen I. Rosenfeld, Mark S. Amonette |
|---|---|
| Rok vydání: | 1993 |
| Předmět: |
Pulmonary and Respiratory Medicine
biology business.industry food and beverages Immunoglobulin E Serum ige Hydrolysate Ige mediated Cow's milk allergy Milk hypersensitivity Pediatrics Perinatology and Child Health biology.protein Immunology and Allergy Medicine Food science Antibody Bovine serum albumin business |
| Zdroj: | Pediatric Asthma, Allergy & Immunology. 7:99-109 |
| ISSN: | 1557-7767 0883-1874 |
| DOI: | 10.1089/pai.1993.7.99 |
| Popis: | SDS-polyacrylamide minigel electrophoresis was performed on cow's milk (CM), four purified bovine proteins (α-casein, α-lactalbumin, β-lactoglobulin, and bovine serum albumin), four CM protein hydrolysate infant formulas (Good Start, Ultra Good Start, Alfare, and Nutramigen), and a soy formula (Isomil). Separated proteins were then electrotransferred to nitrocellulose paper, and immunoblotting for IgE antibodies was performed using the serum from 21 children with IgE-mediated cow's milk hypersensitivity. These children had previously been divided into three groups based on patterns of skin prick test positivity to CM and CM protein hydrolysate formulas: group 1A—positive to CM only, group 1B— positive to CM and whey hydrolysate (Good Start), group 1C—positive to CM, whey hydrolysate (Good Start), and casein hydrolysate (Nutramigen). By history, open challenge, and double-blind, placebo-controlled food challenge (DBPCFC), the children either had topical (group 1A) or systemic (group 1B and 1C) rea... |
| Databáze: | OpenAIRE |
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