Novel Stress Indicating RP-HPLC Method Development and Validation for the Simultaneous Estimation of Ertugliflozin and Sitagliptin in Bulk and its Formulation
| Autor: | Sk. Mastanamma, C. Madhusudhana Chetty, D. China Babu |
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| Rok vydání: | 2018 |
| Předmět: |
Chromatography
Chemistry 010401 analytical chemistry Ornamental horticulture Industrial chemistry General Chemistry 030226 pharmacology & pharmacy 01 natural sciences Biochemistry Method development 0104 chemical sciences Elsevier Biobase Stress (mechanics) 03 medical and health sciences 0302 clinical medicine Sitagliptin Drug Discovery medicine Environmental Chemistry Ertugliflozin Material chemistry medicine.drug |
| Zdroj: | Oriental Journal of Chemistry. 34:2554-2561 |
| ISSN: | 2231-5039 0970-020X |
| DOI: | 10.13005/ojc/340543 |
| Popis: | A selective, sensitive RP-HPLC method was developed for the simultaneous estimation of the Ertugliflozin (ETR) and Sitagliptin (SGT) in bulk and its dosage form. The separation and determination was carried on water’s C18 column capacitate (250X4.6 mm, 5 µm particle size), retention times of Ertugliflozin and Sitagliptin were found to be 2.39 and 4.60 min respectively. The wavelength was fixed at 215nm with PDA detection. The mobile phase was consisted mixture of 0.5 mM potassium dihydrogen ortho phosphate buffer: Methanol in the ratio of 55:45 v/v, pH 5.3 was adjusted with HCl and flow of mobile phase was maintained 1mL/min. The calibration curve was linear and regression co-efficient (R2) value found to be 0.999 and concentration ranging from 37.5-112.5 and 250-750 µg/mL for Ertugliflozin & Sitagliptin respectively. The quantization limit and detection limit of the method were found 0.1 & 0.3 µg/ml and 0.4&1µg/ml for Ertugliflozin & Sitagliptin. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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