Isolation of High-Molecular-Length DNA from Human Skin

Autor:	Haim Hacham, M. Moran, Paula V. Bennett, V S Hejmadi, Betsy M. Sutherland, Swapan K. Ray, R W Gange
Rok vydání:	1996
Předmět:	Human skin Biology Isolation (microbiology) General Biochemistry Genetics and Molecular Biology Sepharose Blot chemistry.chemical_compound Electrophoresis chemistry Biochemistry Separation method Agarose DNA Biotechnology
Zdroj:	BioTechniques. 21:458-463
ISSN:	1940-9818 0736-6205
DOI:	10.2144/96213st03
Popis:	An agarose plug method for isolating high-molecular-length DNA from mammalian tissues has been developed, including from those that are difficult, such as skin. It gives high yields of DNA that contain a minimum of single-strand breaks and is readily digested by restriction and other nucleases. The method requires only simple equipment and is readily adaptable to field or clinical studies.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::7b7b284ceab767d53a94c0a966360e28 https://doi.org/10.2144/96213st03 Zobrazit plný text záznamu