A simple feedback control of Escherichia coli growth for recombinant aldolase production in fed-batch mode
| Autor: | Carles de Mas, Josep López-Santín, Jaume Pinsach |
|---|---|
| Rok vydání: | 2006 |
| Předmět: |
Environmental Engineering
biology Aldolase A Biomedical Engineering lac operon Fructose-bisphosphate aldolase Substrate (chemistry) Bioengineering medicine.disease_cause Fed-batch culture law.invention Biochemistry law Recombinant DNA medicine biology.protein Growth rate Escherichia coli Biotechnology |
| Zdroj: | Biochemical Engineering Journal. 29:235-242 |
| ISSN: | 1369-703X |
| DOI: | 10.1016/j.bej.2006.01.001 |
| Popis: | Fed-batch production of recombinant fuculose-1-phosphate aldolase (FucA) by Escherichia coli XL1 Blue MRF′ (pTrcfuc) has been automated by using a simple feedback specific growth rate control strategy. Non-induced continuous cultures were conducted in order to characterize substrate consumption and carbon dioxide production yields and rates. In fed-batch cultures, substrate feeding rate was adjusted using on-line biomass estimation based on exhaust gas analysis and macroscopic mass balances. Overexpression of recombinant protein induced by isopropyl-β- d -thiogalactopyranoside (IPTG) under trc promoter did not affect significantly the control of specific growth rate during 7 h after induction. Growth and protein production curves were parallel until high level of protein expression started to inhibit cell growth. The proposed specific growth rate control strategy has been successfully applied to both non-induced and induced fed-batch cultures that do not exhibit severe growth rate depression. |
| Databáze: | OpenAIRE |
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