Use of biotinylated probes for detecting sickle cell anemia

Autor:	J J Leary, J T Wilson, G J Garbutt, George S. Schuster, D C Ward
Rok vydání:	1985
Předmět:	biology Biochemistry (medical) Clinical Biochemistry medicine.disease Molecular biology Sickle cell anemia Restriction fragment genomic DNA chemistry.chemical_compound Endonuclease chemistry Biotinylation biology.protein medicine Hemoglobin Gene DNA
Zdroj:	Clinical Chemistry. 31:1203-1206
ISSN:	1530-8561 0009-9147
DOI:	10.1093/clinchem/31.7.1203
Popis:	Earlier, we reported that the 5' end of the normal beta-globin gene (beta) resides on a 1.14-kilobase DNA fragment, whereas the 5' end of the sickle cell gene (beta s) resides on a 1.34-kilobase fragment. In that blot hybridization analysis, we used genomic DNA digested with restricted endonuclease Mst II, and radioactive probes with short half-life. We demonstrate here that, if a biotinylated probe is used instead in a slightly modified procedure, sickle cell anemia can be quickly and directly detected if there is as much as 5 micrograms of total genomic DNA in the sample. This procedure obviates the special precautions necessary when radioactive materials are used.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::7ad2543685a55d358d2da9eae1e94c77 https://doi.org/10.1093/clinchem/31.7.1203 Zobrazit plný text záznamu