Doxorubicin-Bioconjugated Cadmium Sulfide Dextrin Quantum Dots for Imaging Cells
| Autor: | G. Gonzalez De La Cruz, A. Mastache-Juarez, P. Rodríguez-Fragoso, Jorge Reyes-Esparza, L. Rodriguez-Fragoso Lourdes |
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| Rok vydání: | 2020 |
| Předmět: |
Fluorescence-lifetime imaging microscopy
Bioconjugation biology technology industry and agriculture Nanoparticle equipment and supplies biology.organism_classification Cadmium sulfide HeLa chemistry.chemical_compound chemistry Quantum dot Biophysics medicine Doxorubicin Viability assay medicine.drug |
| Zdroj: | Indian Journal of Pharmaceutical Sciences. 82 |
| DOI: | 10.36468/pharmaceutical-sciences.643 |
| Popis: | In this investigation, quantum dots of cadmium sulfide semiconductor crystals capped with dextrin and bioconjugated with doxorubicin were used to target and provide imaging of HeLa cells, which play a critical role in cervical cancer. These dextrin-capped cadmium sulfide nanoparticles/semiconductor quantum dots bioconjugated with doxorubicin are in the range of 5 nm in size and exhibit an intense fluorescence emission in the green and red spectrum, which benefited fluorescence imaging on HeLa cells. Results obtained showed that dextrin-capped cadmium sulfide nanoparticles/semiconductor quantum dots bioconjugated with doxorubicin have a large accumulation time in cells distributed in the cytoplasm and nucleus, where the pharmacological effects of drugs are enhanced. Cells treated with dextrin-capped cadmium sulfide nanoparticles/semiconductor quantum dots bioconjugated with doxorubicin also showed a major increase in size, including cytoplasm and nucleus, as compared to cells treated with doxorubicin alone. Additionally, binucleated cells and membrane blebbing was also found. Therefore, dextrin-capped cadmium sulfide nanoparticles/semiconductor quantum dots bioconjugated with doxorubicin could be used as probes for simultaneous cell cancer-targeted fluorescent imaging, tracking and monitoring cell viability with the advantage of improving the clinical effects of antineoplastic drugs with increasing apoptosis. |
| Databáze: | OpenAIRE |
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