Detection of Free Prostate-Specific Antigen Using a Novel Single-Chain Antibody (scAb)-Based Magneto-Immunosensor
| Autor: | Julia Zapatero-Rodríguez, Richard O'Kennedy, Susana Liébana, Shikha Sharma, Guido A. Drago, Sarah Gilgunn |
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| Rok vydání: | 2017 |
| Předmět: |
biology
Chemistry 010401 analytical chemistry Biomedical Engineering Substrate (chemistry) Bioengineering 02 engineering and technology Conjugated system 021001 nanoscience & nanotechnology 01 natural sciences Horseradish peroxidase Primary and secondary antibodies Molecular biology Amperometry 0104 chemical sciences Antigen biology.protein Antibody 0210 nano-technology Biosensor |
| Zdroj: | BioNanoScience. 8:680-689 |
| ISSN: | 2191-1649 2191-1630 |
| DOI: | 10.1007/s12668-017-0394-2 |
| Popis: | The proportion of prostate-specific antigen (PSA) circulating in the free form can help to differentiate prostate cancer from benign prostate disease. In this paper, novel highly specific single-chain antibody (scAb) fragments were conjugated to magnetic particles (MPs) for the recognition and magnetic separation of free PSA (fPSA). A sandwich-type immunoassay method was developed, in which fPSA was captured by the scAb-conjugated MPs and labelled by a secondary antibody, conjugated to horseradish peroxidase (HRP) enzyme. The biosensor was constructed on carbon screen-printed electrodes (SPEs) modified with magnetic particles for increased surface area and fast reaction kinetics, thus enhancing binding capacity. The amperometric response was measured at −0.15 V, using hydrogen peroxide as the HRP substrate and hydroquinone (HQ) as the electrochemical mediator. The magneto-immunosensor demonstrated a sensitivity of 0.5 ng mL−1, with a linear detection range up to 10 ng mL−1 of fPSA. |
| Databáze: | OpenAIRE |
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