Monoamine Oxidase Activity in Larvae of the European Corn Borer13

Autor:	D. K. Hayes, D. B. Wash, Milton S Schechter
Rok vydání:	1972
Předmět:	European corn borer Larva Ecology biology Heliothis virescens Monoamine oxidase fungi Substrate (chemistry) General Medicine biology.organism_classification Ostrinia Biochemistry Insect Science Centrifugation Homogenization (biology)
Zdroj:	Journal of Economic Entomology. 65:1229-1232
ISSN:	1938-291X 0022-0493
DOI:	10.1093/jee/65.5.1229
Popis:	Monoamine oxidase (MAO) activity was determined in homogenates of larvae and pupae of the European corn borer, Ostrinia nubilalis (Hubner), and the tobacco bud worm, Heliothis virescens (F.), by using 214C-tryptamine as a substrate. Optimum activity was produced by addition of dithiotbreitol to the homogenizing medium and by removing the oxygen from this solution with nitrogen before homogenization. More than 80% of the activity was found in the supernatant fraction of the homogenate resulting from centrifugation at 105,000-g equivalents. Activity was inhibited by addition of s -triazines and by some known MAO inhibitors. Homogenates prepared at different times of day had different levels or activity.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::7a6e54950bc202b93ea3ac6852d30349 https://doi.org/10.1093/jee/65.5.1229 Zobrazit plný text záznamu