Mouse Fetal Germ Cell Isolation and Culture Techniques
| Autor: | Josephine Bowles, Cassy M. Spiller, Guillaume Burnet |
|---|---|
| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
endocrine system Cell signaling Fetus Gonad medicine.diagnostic_test Cell Biology Isolation (microbiology) Molecular biology Flow cytometry 03 medical and health sciences 030104 developmental biology 0302 clinical medicine medicine.anatomical_structure embryonic structures medicine Germ 030217 neurology & neurosurgery Germ cell |
| Zdroj: | Methods in Molecular Biology ISBN: 9781493940158 |
| DOI: | 10.1007/978-1-4939-4017-2_13 |
| Popis: | The fetal gonad contains a great variety of differentiating cell populations, of which germ cells make up a small percentage. In order to study germ cell-specific gene and protein expression, as well as determine direct effects of signaling molecules, it is necessary to prepare enriched populations of germ cells and maintain them in culture for several hours to multiple days. The protocols in this chapter are designed to provide a guide for the isolation or enrichment of mouse primordial germ cells (from 9.5 days postcoitum (dpc) to 18.5 dpc) by flow cytometry (Subheading 3.1) or magnetic sorting (Subheading 3.2), followed by primary germ cell culture (Subheading 3.3). |
| Databáze: | OpenAIRE |
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