Cytokine mRNA abundance in intestinal biopsies from dogs with chronic diarrhea
| Autor: | J.W. Blum, S.N. Sauter, Karin Allenspach |
|---|---|
| Rok vydání: | 2007 |
| Předmět: |
General Veterinary
040301 veterinary sciences business.industry 0402 animal and dairy science Interleukin 04 agricultural and veterinary sciences medicine.disease 040201 dairy & animal science Inflammatory bowel disease 0403 veterinary science Diarrhea medicine.anatomical_structure Real-time polymerase chain reaction Elimination diet Immunology medicine Duodenum Prednisolone Tumor necrosis factor alpha medicine.symptom business medicine.drug |
| Zdroj: | Veterinární medicína. 52:353-364 |
| ISSN: | 1805-9392 0375-8427 |
| DOI: | 10.17221/1876-vetmed |
| Popis: | The differentiation between inflammatory bowel disease (IBD) and food responsive diarrhea (FRD) is difficult and no objective markers are available. We postulated that patterns of selected key cytokines would help to objectively differentiate between the two subcategories of chronic enteropathies in dogs. We studied mRNA patterns of selected cytokines in dogs with chronic enteropathies. Ten dogs with FRD (= group FRDbef ) and seven dogs with IBD (= group IBDbef ) were presented for endoscopy at the Small Animal Clinic, University of Bern. A control endoscopy was performed in both groups after treatment with an elimination diet for four weeks (FRDaft) or with an elimination diet combined with prednisolone for 10 weeks (IBDaft). Intestinal control samples of gastrointestinally healthy dogs from an independent study were additionally available. Dogs were clini - cally examined and scored using the canine IBD activity index (CIBDAI). mRNA abundance of interleukin (IL)-5, -10, -12p40, and -13, tumor necrosis factor (TNF)-α, transforming growth factor (TGF)-β1, and interferon (IFN)-γ were analyzed in intestinal samples by reverse transcription and real time polymerase chain reaction. Median CIBDAI decreased in FRDaft ( P < 0.01) and IBDaft ( P = 0.07) during treatment. In duodenum, IL-12p40 mRNA |
| Databáze: | OpenAIRE |
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