Production of penicillin in a fluidized-bed bioreactor using urethane foams as carriers

Autor:	Kenji Tachi, Tetsuo Kobayashi, Isao Endo, Teruyuki Nagamune
Rok vydání:	1990
Předmět:	Mass transfer coefficient Chromatography Superficial velocity biology Chemistry General Chemical Engineering General Chemistry Penicillium chrysogenum biology.organism_classification Penicillin Defoamer Volume (thermodynamics) Fluidized bed Bioreactor medicine medicine.drug
Zdroj:	JOURNAL OF CHEMICAL ENGINEERING OF JAPAN. 23:408-413
ISSN:	1881-1299 0021-9592
Popis:	By using urethane foams as immobilizing carriers for Penicillium chrysogenum, the effect of apparent volumetric mass transfer coefficient of oxygen kLa on penicillin production in a fluidized-bed bioreactor (FBR) was investigated. The kLa value was measured by varying the amount of urethane foam carriers added as well as aeration rate, suspended cell mass concentration and antifoam reagent concentration in a model cultivation system. High production of penicillin by batch operation was obtained in the operational region where the volumetric ratio of carriers in the working volume of FBR was 0.3 to 0.4 and gas superficial velocity was 0.03 to 0.05 m·s–1. Under these fluidizing operational conditions, a repeated batch operation was performed. The adhered cells in the carriers could be used repeatedly for semicontinuous production of penicillin, and a penicillin yield of about twice the quantity of lactose utilized could be obtained.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::7a28140b4a3cd36cdec60e483b820b52 https://doi.org/10.1252/jcej.23.408 Zobrazit plný text záznamu