In vitro effect of fosfomycin on multi-drug resistant gram-negative bacteria causing urinary tract infections
| Autor: | Mailan Natarajan, Pallam Gopichand, Jharna Mandal, Girija Agarwal, LN Dorairajan, Surendran Deepanjali, Sreejith Parameswaran |
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| Rok vydání: | 2019 |
| Předmět: |
0301 basic medicine
Pharmacology biology medicine.drug_class Klebsiella pneumoniae 030106 microbiology Antibiotics Enterobacter biochemical phenomena metabolism and nutrition Fosfomycin Antimicrobial biology.organism_classification Enterobacteriaceae Meropenem Microbiology 03 medical and health sciences 0302 clinical medicine Infectious Diseases Nitrofurantoin medicine Pharmacology (medical) 030212 general & internal medicine medicine.drug |
| Zdroj: | Infection and Drug Resistance. 12:2005-2013 |
| ISSN: | 1178-6973 |
| Popis: | Background Rising rates of resistance to antimicrobial drugs among Enterobacteriaceae limit the choice of therapeutic agents to treat urinary tract infections. In this context we assessed the in-vitro effect of fosfomycin against extended-spectrum beta-lactamases, AmpC beta-lactamases and carbapenemase-producing strains of Escherichia coli, Klebsiella pneumoniae, Enterobacter spp, and P seudomonas aeruginosa isolated from the patients with urinary tract infection (UTI) and also studied the effect of fosfomycin on their biofilm formation. Materials and methods A total of 326 multidrug-resistant (MDR) isolates comprising of Escherichia coli, Klebsiella pneumoniae, Enterobacter spp, and P seudomonas aeruginosa from the urine samples of the patients with a diagnosis of UTI were included in the study. MIC 50 and MIC 90 were detected by agar dilution method and the capacity to form biofilm in the presence of fosfomycin by these MDR isolates was assessed by the tissue culture plate method. Results The MIC50 for meropenem (0.5 µgm/mL) and nitrofurantoin (32 µgm/mL) was within the susceptible range only for E. coli. Fosfomycin was the only antibiotic that inhibited 100% E.coli, 70% Klebsiella spp, and 50% Pseudomonas spp and 40% Enterobacter spp which included the extended-spectrum beta-lactamases producers. It showed a similar effect on carbapenemase producers and AmpC producers. Fosfomycin disrupted biofilm in 67% (n=141) E.coli, 74% (n=50) Klebsiella spp, 88% (n=27) Pseudomonas spp and 36% (n=23) Enterobacter spp at 24 hrs of incubation with a concentration of 2 fold dilution lower than that of the MIC. Conclusion Fosfomycin showed a good inhibitory effect on the biofilms produced by the MDR organisms studied here. |
| Databáze: | OpenAIRE |
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