Some cationic porphyrins: synthesis, stabilization of G-quadruplexes, and down-regulation of c-myc in Hep G2 cells

Autor:	Lin Liu, Dan Zhang, Shao R. Wang, Xiao L. Zhang, Xiao C. Weng, Jing Huang, Guo R. Li, Xiang Zhou, Feng L. Luo
Rok vydání:	2009
Předmět:	Circular dichroism Oncogene biology Cationic polymerization General Chemistry G-quadruplex Reverse transcription polymerase chain reaction Hep G2 chemistry.chemical_compound chemistry Biochemistry biology.protein heterocyclic compounds DNA Polymerase
Zdroj:	Journal of Porphyrins and Phthalocyanines. 13:865-875
ISSN:	1099-1409 1088-4246
DOI:	10.1142/s1088424609001121
Popis:	The stabilization of G-quadruplex DNA represents an attractive strategy for the design and development of novel antitumor drugs. In the present work, we have designed and synthesized nine cationic porphyrins, each with four side arms at their meso positions. The interactions of these porphyrins with both human telomeric DNA and NHE III1 G-quadruplexes were measured by various DNA binding assays, including polymerase stop assay, circular dichroism (CD) and CD melting assay. We then proceeded to investigate their effects on the expression of c-myc oncogene in the Hep G2 cell line. The experimental results indicate that these porphyrins are capable of effectively inducing or stabilizing both human telomeric and NHE III1 G-quadruplexes in the presence or absence of metal ions. Furthermore, we have discovered that porphyrins with a stronger stabilizing effect on c-myc G-quadruplexes lead to more pronounced down-regulation of the c-myc oncogene in the Hep G2 cell line.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::79e8f527828baef78d6a8e56ff8f3d3b https://doi.org/10.1142/s1088424609001121 Zobrazit plný text záznamu