DNA‐polymerase α, β, δ and ε activities in isolated neuronal and astroglial cell fractions from developing and aging rat cerebral cortex

Autor: Kalluri Subba Rao, N.S Raji, T Hari Krishna
Rok vydání: 2002
Předmět:
Zdroj: International Journal of Developmental Neuroscience. 20:491-496
ISSN: 1873-474X
0736-5748
DOI: 10.1016/s0736-5748(02)00079-5
Popis: The relative proportions of DNA-polymerases alpha, beta, delta and epsilon (pols alpha, beta, delta and epsilon ) activities in isolated neuronal and astroglial cell fractions from developing, adult and aging rat brain cerebral cortex, were examined. This was achieved through a protocol that takes advantage of the reported differential sensitivities of different DNA-polymerases towards certain inhibitors like butylphenyl and butylanilino nucleotide analogs, 2',3'-dideoxythymidine triphosphate (ddTTP), monoclonal antibody of human alpha polymerase and the use of two template primers as substrates. The results indicate that while DNA-polymerase beta (pol beta) is the predominant enzyme, significant levels of DNA-polymerases alpha and delta/epsilon (pols alpha and delta/epsilon ) are also present in both cell types at all the post-natal ages studied. A notable difference regarding the relative abundance of DNA-polymerases other than beta is the higher percentage of pol delta/epsilon in neurons and a more sustained pol alpha activity through the life span in astroglia. The presence of detectable proportion of DNA-polymerases other than beta (particularly the delta/epsilon type) may be taken to indicate their role in long patch base excision repair as well as in other modes of DNA repair.
Databáze: OpenAIRE
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