Abstract WP533: Chronic Cerebral Damage Aggravates Vascular Remodeling in Cuff-Induced Vascular Injury Mouse Model
| Autor: | Masatsugu Horiuchi, Hui-Yu Bai, Masaki Mogi, Akinori Higaki, Li-Juan Min, Masanori Kukida, Bao-Shuai Shan, Kana Tsukuda, Hirotomo Nakaoka, Jun Iwanami |
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| Rok vydání: | 2019 |
| Předmět: |
Advanced and Specialized Nursing
medicine.medical_specialty business.industry Organ dysfunction Cardiorenal syndrome medicine.disease nervous system Valvular disease Internal medicine Ischemic stroke Cuff cardiovascular system medicine Cardiology cardiovascular diseases Neurology (clinical) Cerebral damage medicine.symptom Cardiology and Cardiovascular Medicine business |
| Zdroj: | Stroke. 50 |
| ISSN: | 1524-4628 0039-2499 |
| Popis: | Introduction: Distant organ dysfunction has been highlighted whereby acute or chronic damage in one organ may induce dysfunctions of the other organs such as cardiorenal syndrome. Vascular injury is an important process in various vascular diseases. However, the relationship cerebral ischemic damage and peripheral vascular disorder is still unclear. Therefore, we have investigated the possible pathophysiological interactions between brain ischemia and vascular remodeling. Methods: Eight-week-old male C57BL/6 mice underwent permanent cerebral brain ischemia by permanent occlusion of the left middle cerebral artery occlusion (MCAO) by electrocoagulation using a subtemporal approach. Four weeks after MCAO, vascular injury was induced by polyethylene cuff placement on the femoral artery. Neointima formation was determined 14 days after cuff placement by evaluating intima/media ratio. Mice were administrated 0.5% tempol, antioxidant agent, in drinking water from after MCAO. Macrophage fractions in spleen two weeks after MCAO were examined and M1 and M2-polarized macrophages were separated by flow cytometry and analyzed. Results: Cerebral ischemia did not influence the intima/media ratio 6 weeks after MCAO compared with sham-operated mice. Neointima formation in the injured artery was significantly increased 2 weeks after cuff placement. Interestingly, the neointima formation 2 weeks after cuff placement was more marked in MCAO-operated mice compared with sham-operated mice. We observed that treatment with tempol did not improve increased neointima formation in MCAO-operated mice. Next, we examined the macrophage fractions in spleen 2 weeks after MCAO. The macrophage fraction in SVF evaluated by F4/80 staining did not differ between sham- and MCAO-operated mice. There was no significant difference in M1 and M2 fraction with/without MCAO. Conclusion: These results suggest that cerebral ischemic injury aggravate peripheral vascular disorders, and we are addressing the possible roles of renin-angiotensin system and inflammation etc. involved in these interesting results, which could provide us with the discovery of new pathological mechanisms concerning the distant organ dysfunctions between brain and peripheral vasculature. |
| Databáze: | OpenAIRE |
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