A differential proteomic study reveals the downregulation of several plasma membrane Ca2+-binding proteins in the sperm of Atlantic salmon (Salmo salar) following cold storage

Autor: Juan-Alejandro Norambuena, Jorge F. Beltrán, Iván Valdebenito, Jennie Risopatrón, Joaquín A. Farias, Lisandra Herrera Belén, Mauricio Zamorano, Jorge G. Farías
Rok vydání: 2021
Předmět:
Zdroj: Aquaculture. 545:737211
ISSN: 0044-8486
DOI: 10.1016/j.aquaculture.2021.737211
Popis: Sperm motility is a key event in fertilization, which is regulated by different factors. Among the factors that most affect sperm motility in fish are the external concentrations of Ca2+, the influence of which is highly regulated by various plasma membrane Ca2+-binding proteins. Concentrations of this cation have also been shown to be one of the most important factors affecting motility in cold-stored sperm cells. Taking into account the aforementioned aspects, we carried out a differential proteomic study on Salmo salar sperm stored at 4 °C with the aim of evaluating the protein profile on day 0 and day 14. The results of our study showed that 401 proteins were significantly downregulated (p
Databáze: OpenAIRE