Application of an Environmental Phage-Based Assay (Sample6 Detect HT/L) for the Detection of Listeria spp. in Ice Cream
| Autor: | Brandon Selover, Sam Anderson, Jasmine M Pando, Lourdes M Nadala, Mansour Samadpour, Tam Mai, Elba Veronica Arias-Rios, Kristina Tenney, Seana K Davidson, Ruth Marie Cantera |
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| Rok vydání: | 2019 |
| Předmět: |
Pharmacology
biology 010405 organic chemistry Chemistry 010401 analytical chemistry Early detection medicine.disease_cause biology.organism_classification 01 natural sciences 0104 chemical sciences Analytical Chemistry Incubation period Food and drug administration Listeria monocytogenes Ice cream medicine Listeria Environmental Chemistry Food science Agronomy and Crop Science Food Science |
| Zdroj: | Journal of AOAC INTERNATIONAL. 102:1132-1137 |
| ISSN: | 1944-7922 1060-3271 |
| DOI: | 10.5740/jaoacint.18-0253 |
| Popis: | Background: Dairy products are common sources of Listeria outbreaks, and early detection of the pathogen is critical to prevent outbreaks of illnesses and financial losses for dairy producers. Objective: This study aimed to evaluate Sample6 Detect HT/L for effective detection of Listeria monocytogenes and L. innocua in ice cream. Methods: Performance of the Sample6 DETECT HT/L was compared with U.S. Food and Drug Administration (FDA) Bacteriological Analytical Manual (BAM) Chapter 10 method for detection of Listeria spp. in ice cream using an unpaired study design. Results: R2-enriched samples tested with Sample6 Detect HT/L performed as well as the reference method at all time points tested from 15 to 24 h. R2 is a proprietary blend for use with the test kit that helps with early detection. All the dPODC values (Sample6 Detect HT/L presumptive and confirmed results) equaled zero, indicating 100% concordance between the methods. Both Sample6 Detect HT/L and FDA BAM results showed low dPODC values, with confidence intervals indicating no significant differences between Sample6 Detect HT/L and reference method results. Conclusions: Sample6 Detect HT/L is suitable to detect Listeria spp. in ice cream, even with a 12 h enrichment. Sample6 Detect HT/L demonstrated equivalent detection of L. monocytogenes and L. innocua from R2-enriched samples as expected with 15 and 18 h enrichment when compared with the 24 h FDA BAM method for L. monocytogenes. Highlights: These results indicate that Sample6 Detect HT/L, primarily developed for environmental samples, can be used to detect Listeria spp. in ice cream with less incubation time, resulting in faster detection. |
| Databáze: | OpenAIRE |
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