C2-ceramide inhibits LPS-induced nitric oxide production in RAW 264.7 macrophage cells through down-regulating the activation of Akt

Autor:	Isamu Mori, Takashi Yokochi, Tomoaki Yoshida, Mya Mya Mu, Naoki Koide, Tsuyoshi Sugiyama
Rok vydání:	2003
Předmět:	Ceramide Lipopolysaccharide Kinase Immunology Cell Biology Transfection Microbiology Molecular biology Nitric oxide chemistry.chemical_compound Infectious Diseases chemistry Biochemistry Cell culture Phosphorylation lipids (amino acids peptides and proteins) Molecular Biology Protein kinase B
Zdroj:	Journal of Endotoxin Research. 9:85-90
ISSN:	0968-0519
DOI:	10.1179/096805103125001450
Popis:	The effect of C(2)-ceramide, a membrane-permeable ceramide analogue, on nitric oxide (NO) production in lipopolysaccharide (LPS)-stimulated RAW 264.7 cells was studied. The non-toxic concentration of C(2)-ceramide inhibited LPS-induced NO production. It was due to the attenuated expression of the inducible type of NO synthase (iNOS). C(2)-ceramide did not influence the phosphorylation of a series of mitogen-activated protein (MAP) kinases in response to LPS. On the other hand, C(2)-ceramide down-regulated the phosphorylation of Akt in LPS-stimulated RAW 264.7 cells, followed by the impairment of nuclear factor (NF)-kappaB activation. Moreover, the Akt dominant-negative mutant inhibited LPS-induced NO production. C(2)-ceramide was suggested to inhibit LPS-induced NO production through down-regulating the activation of Akt.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::782e76c1a52439562928c91a8575f90d https://doi.org/10.1179/096805103125001450 Zobrazit plný text záznamu Plný text ve formátu PDF