Characterization of enzymatically isolated myocytes from the turtle, Chrysemys picta

Autor:	Kurt H. Lustig, A. Martin Gerdes, Joseph M. Capasso
Rok vydání:	1996
Předmět:	medicine.medical_specialty Physiology Cell volume Turtle (syntax) Isolated myocytes Biology Biochemistry Sarcomere Endocrinology Internal medicine medicine Biophysics Myocyte Cell isolation Molecular Biology
Zdroj:	Comparative Biochemistry and Physiology Part B: Biochemistry and Molecular Biology. 115:457-464
ISSN:	1096-4959
DOI:	10.1016/s0305-0491(96)00117-4
Popis:	Application of an enzymatic cell isolation technique to a turtle heart yielded mononuclear spindle-shaped myocytes. Turtle myocyte morphology revealed a long, thin, tapered cell with an average length of 221± 9.6 μm and an average width of 9.55 ± 0.87 μm. Cell volume was calculated to be 16,248 ± 24,776 μm3 and mean sarcomere length was 2.24 ± 0.09 μm. The Ca+2-tolerant myocytes shortened to a degree of 13 ± 6.1% when stimulated in an electric field. Both spontaneous and induced contractions resembled a twitch. Myocyte volume did not vary significantly with the body mass of the turtle.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::7767667d59d8f74cbce9c913058ae477 https://doi.org/10.1016/s0305-0491(96)00117-4 Zobrazit plný text záznamu Full Text from ScienceDirect