Extraction, Purification and Antioxidant Activity of Intracellular Polysaccharides from a Strain of Lachnum sp

Autor: Jing Min Cai, Ru Yue Yuan, Tao Qiu, Ming Ye
Rok vydání: 2011
Předmět:
Zdroj: Advanced Materials Research. :2114-2117
ISSN: 1662-8985
DOI: 10.4028/www.scientific.net/amr.236-238.2114
Popis: The microwave method was used to extract Lachnum calyculiforme polysaccharides (LCP). By orthogonal experiments, the optimal conditions of extraction were the microwave temperature was 120 °C, extraction time was 20 min, ratio of water to raw material was 60:1, and the yield of polysaccharides was 12.56 %. LCP-1 was separated and sequentially purified from LCP through Sephadex G-100 column chromatography, which was detected as the homogeneous component. LCP -1 had strong scavenging abilities on O2ˉ•, •OH and DPPH free radicals.
Databáze: OpenAIRE