A comparison of time-resolved fluoroimmunoassay and ELISA in the detection of casein in foodstuffs

Autor:	Eliann Egaas, H.-J. Skarpeid, Gaynour B.G. Sletten, Lena Haugland Moen, Kjersti E. Lovberg
Rok vydání:	2005
Předmět:	Streptavidin Detection limit Analyte medicine.diagnostic_test biology Chemistry Immunology Immunoelectrophoresis Primary and secondary antibodies Standard curve chemistry.chemical_compound Casein Biotinylation medicine biology.protein Food science Agronomy and Crop Science Food Science
Zdroj:	Food and Agricultural Immunology. 16:235-243
ISSN:	1465-3443 0954-0105
Popis:	With the aim of lowering the detection limit for casein in foods, three competitive assays are described: direct time-resolved fluoroimmunoassay (TR-FIA), using europium-conjugated antibody, indirect TR-FIA, using biotinylated antibody with europium-conjugated streptavidin and ELISA, using a HRP-conjugated secondary antibody. Food samples (instant potato, flour mix, packet soup, spice-mix) were analysed. Standard curve sensitivities in direct and indirect TR-FIAs did not differ significantly (p=0.097), but both TR-FIAs were considerably less sensitive (both p
Databáze:	OpenAIRE
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