Visualization and analysis of actin cytoskeleton organization in plants
| Autor: | G. A. Pozhvanov |
|---|---|
| Rok vydání: | 2018 |
| Předmět: |
biology
Cytoskeleton organization Chemistry macromolecular substances Microfilament General Biochemistry Genetics and Molecular Biology Actin cytoskeleton organization Cell biology Cytoplasmic streaming Microtubule biology.protein Actin-binding protein General Agricultural and Biological Sciences Cytoskeleton Actin |
| Zdroj: | Biological Communications. 63:59-69 |
| ISSN: | 2587-5779 2542-2154 |
| Popis: | The plant cytoskeleton is a highly dynamic system that consists of two components: microfilaments and microtubules. Actin microfilaments are essential for polar growth, cytoplasmic streaming, directing polar growth, anchoring the nucleus, gravity sensing, signalling pathway integration and a number of other functions. Actin morphology and dynamics are orchestrated by a variety of small actin binding proteins, and some of them have become a source of actin interaction domains widely used as markers for microfilaments in fusions with fluorescent reporter proteins. However, older techniques are still employed for actin visualization. In this short review, we will focus on the diversity of fluorescent reporter fusions for F-actin and on approaches and existing free software for the analysis of cytoskeleton organization, mainly in Arabidopsis. Abbreviations: MF ― microfilament, MT ― microtubule, GFP ― green fluorescent protein, MFA ― Microfilament Analyzer. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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