Hydrogen peroxide-induced microsatellite instability in the K-12 endogenous gene

T:A, G:C-->C:G, and A:T-->T:A). The types and the nature of the mutations did not differ between strains. Frameshift mutations occurred at G:C and A:T sites equally, and in repeated and non-repeated sequences equally. It is evident that endogenous oxidative damage to DNA can increase the frequency of strand slippage intermediates occurring during DNA replication and contribute to genomic instability. Our results further indicate that oxyR regulon does not take part in the DNA-repair pathway against oxidative damage induced by hydrogen peroxide. -->

ISSN:	0006-291X
Přístupová URL adresa:	https://explore.openaire.eu/search/publication?articleId=doi_________::7573a2e9f7881ba4d843f31d71d65e0e https://doi.org/10.1016/s0006-291x(03)01027-1
Rights:	CLOSED
Přírůstkové číslo:	edsair.doi...........7573a2e9f7881ba4d843f31d71d65e0e
Autor:	Eiji Yamamura, Kazuo Yamamoto, Tatsuo Nunoshiba, Takehiko Nohmi
Rok vydání:	2003
Předmět:	Genome instability chemistry.chemical_classification Reactive oxygen species Mutation DNA repair Mutagenesis Biophysics Cell Biology Biology medicine.disease_cause Biochemistry Molecular biology Frameshift mutation chemistry.chemical_compound chemistry medicine bacteria Hydrogen peroxide Molecular Biology Escherichia coli
Zdroj:	Biochemical and Biophysical Research Communications. 306:570-576
ISSN:	0006-291X
Popis:	Damage to DNA by reactive oxygen species may be a significant source of endogenous mutagenesis in aerobic organisms. Using an endogenous tonB gene as a mutation selective marker in Escherichia coli, we have examined whether endogenous oxidative mutagenesis can contribute to genetic instability. We have also used oxyR(+) and oxyR(-) strains to evaluate how hydrogen peroxide scavenging system can contribute to genetic instability. The highest mutation frequency induced by hydrogen peroxide was 3.8x10(-6) at 600 microM and 5.3 x 10(-6) at 40 microM in oxyR(+) and oxyR(-), respectively. Hydrogen peroxide induced minus frameshift mutations predominantly followed by transversions (G:C-->T:A, G:C-->C:G, and A:T-->T:A). The types and the nature of the mutations did not differ between strains. Frameshift mutations occurred at G:C and A:T sites equally, and in repeated and non-repeated sequences equally. It is evident that endogenous oxidative damage to DNA can increase the frequency of strand slippage intermediates occurring during DNA replication and contribute to genomic instability. Our results further indicate that oxyR regulon does not take part in the DNA-repair pathway against oxidative damage induced by hydrogen peroxide.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::7573a2e9f7881ba4d843f31d71d65e0e https://doi.org/10.1016/s0006-291x(03)01027-1 Zobrazit plný text záznamu Full Text from ScienceDirect