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Objective Chemotherapy resistance and side effects are important reasons for the failure of lung cancer treatment. Therefore, finding new sensitizers for chemotherapeutic drugs is an urgent problem to be solved.Method In this study, A549 cells were given different pharmacological interventions, including control, cisplatin, DMY and the combination of cisplatin and DMY. The level of cell proliferation and apoptosis were detected by MTT assay and Flow cytometry AV/PI double staining. Transwell assay was adopted to detect the ability of migration and invasion of A549 cells. Western blot analyzed the expression of protein about proliferation, apoptosis, migration, and invasion.Results The present study denoted that DMY strengthened the effect of cisplatin on the inhibition of proliferation in lung cancer A549 cells. Meanwhile, DMY promoted cisplatin induced apoptosis of A549 cells. Further, DMY combined with cisplatin can synergistically inhibit the migration and invasion of A549 cells. Western blotting results showed that the expression of E-cadherin was significantly increased in the combination group compared to cisplatin group, while, the expression of N-cadherin, matrix metalloproteinase MMP 2, MMP 9 and Smads proteins (p-SMAD 3, t-SMAD 3, t-SMAD 4), were significantly decreased in the combination group.Conclusion Low dosage of DMY can significantly enhance the effect of cisplatin treatment in lung cancer cells, and its mechanism may be related to the induction of apoptosis, inhibition of proliferation, migration and invasion, which is expected to be a low-toxic and efficient chemosensitizer for lung cancer treatment. |
