Theory and Applications of Confocal Microscopy

Autor: Irene L. Hale, Brian Matsumoto, Theresa R. Kramer
Rok vydání: 1996
Předmět:
Zdroj: Analytical Morphology ISBN: 9781461286837
DOI: 10.1007/978-1-4612-4166-9_11
Popis: Light microscopy is undergoing a rapid development as a research tool for the cell biologist. The recent integration of computers and optics has provided cell biologists the tools for measuring physiological processes, for studying structures that are much smaller than the wavelength of light and for reconstructing the three-dimensional architecture of tissues. An instrument type that has greatly benefited from this integration is the confocal microscope. This instrument uses an intense beam of light to raster across the specimen; it then integrates the scans to form an image. Since the effect of light scatter is minimized, the micrograph is of very high contrast. Many of these microscopes use a computer to control the illuminating light and to digitally enhance the data. These attributes enable the microscopist to study and analyze cellular processes and structures that are beyond the capabilities of traditional light and electron microscopy.
Databáze: OpenAIRE