| Autor: |
Shahla Nemati, Pawel S. Jung, Demetri N. Christodoulides, Scott E. Baker, Nava R. Subedi, Andreas E. Vasdekis, Erin L. Bredeweg |
| Rok vydání: |
2021 |
| Předmět: |
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| Zdroj: |
Quantitative Phase Imaging VII. |
| DOI: |
10.1117/12.2583926 |
| Popis: |
We report the integration of quantitative-phase imaging (QPI) with light-sheet (LS) fluorescent microscopy on to a standard inverted microscope that retains compatibility with microfluidics. QPI enables label-free imaging and number-density quantification of single cells and their organelles. Conversely, LS yields considerable speed and phototoxicity gains in quantifying the 4D dynamics of gene-encoded fluorescent biomarkers. We will detail the system design that relied on spatial light interferometry for QPI and an accelerating Airy-beam light-sheet for fluorescence, its performance, as well as results of a representative multivariate imaging analysis of single-cell metabolism. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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