| Autor: |
Juan Passas, Almudena Albillos, Nuria García-Magro, Cristina de Castro-Guerín, Sergio Alonso y Gregorio, Jose Carlos Caba-González, Alicia Hernández-Vivanco, Beatriz Carmona-Hidalgo, Alberto Perez-Alvarez, Sara Sanz-Lázaro, Jesús Blázquez, Amanda Jiménez-Pompa, Angel Tabernero, Carmen González-Enguita |
| Rok vydání: |
2017 |
| Předmět: |
|
| Zdroj: |
European Journal of Pharmacology. 796:115-121 |
| ISSN: |
0014-2999 |
| DOI: |
10.1016/j.ejphar.2016.12.009 |
| Popis: |
The present study was performed to evaluate the Ca v 1 channel subtypes expressed in human chromaffin cells and the role that these channels play in exocytosis and cell excitability. Here we show that human chromaffin cells obtained from organ donors express Ca v 1.2 and Ca v 1.3 subtypes using molecular and pharmacological techniques. Immunocytochemical data demonstrated the presence of Ca v 1.2 and Ca v 1.3 subtypes, but not Ca v 1.1 or Ca v 1.4. Electrophysiological experiments were conducted to investigate the contribution of Ca v 1 channels to the exocytotic process and cell excitability. Ca v 1 channels contribute to the exocytosis of secretory vesicles, evidenced by the block of 3 μM nifedipine (36.5±2%) of membrane capacitance increment elicited by 200 ms depolarizing pulses. These channels show a minor contribution to the initiation of spontaneous action potential firing, as shown by the 2.5 pA of current at the threshold potential (−34 mV), which elicits 10.4 mV of potential increment. In addition, we found that only 8% of human chromaffin cells exhibit spontaneous action potentials. These data offer novel information regarding human chromaffin cells and the role of human native Ca v 1 channels in exocytosis and cell excitability. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
|
Full Text from ScienceDirect