| Autor: |
Yun-Ling Luo, Yu-Fen Huang, Shih-Tsung Kang, Chih-Kuang Yeh |
| Rok vydání: |
2012 |
| Předmět: |
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| Zdroj: |
2012 IEEE International Ultrasonics Symposium. |
| Popis: |
A novel platform based on gold nanoparticles (Au NPs) coupled with double-stranded DNA sequences and aptamers (referred to as ds(sgc8c)-Au NPs) was developed for ultrasoundcontrolled targeted drug delivery. The sgc8c aptamers with high affinity to CCRF-CEM (T-cell leukemia) cells facilitated the cellular uptake of ds(sgc8c)-Au NPs. The aptamers were extended with repeated CG base pairs for loading anticancer drugs doxorubicin (Dox). After incubated with ds(sgc8c)-Au NPs for 2 h, the cells were irradiated by 10-MHz high-intensity focused ultrasound (HIFU) at a focal spatial-average temporal-average intensity of 140 W/cm2 for 10 min. It was found that the inertial cavitation induced by HIFU drove the specific release of Dox molecules from ds(sgc8c)-Au NPs as indicated by the increase in Dox fluorescence. The temperature within the focal volume only increased by 3°C during the irradiation, but reducing the gas content in the cell medium resulted in a 50% decrease in the fluorescence enhancement. This system can serve as a novel ultrasoundresponsive therapeutic system, showing great promise in targeted drug delivery for remote control of high spatial/temporal resolution as well as reductions in undesirable side effects. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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