Fingerprint Analysis of Codonopsis Radix by HPLC Coupled with Chemometrics Analysis

Autor:	Huan-yang Qi, Yan-Ping Shi, Fang-Fang Chen
Rok vydání:	2013
Předmět:	Pharmacology Chromatography biology Chemistry biology.organism_classification High-performance liquid chromatography Quantitative determination Chemometrics Complementary and alternative medicine Fingerprint Principal component analysis Pharmacology (medical) Statistical analysis Radix Codonopsis
Zdroj:	Chinese Herbal Medicines. 5:307-312
ISSN:	1674-6384
DOI:	10.1016/s1674-6384(13)60046-5
Popis:	Objective To establish a validated high performance liquid chromatography (HPLC) for the fingerprint analysis of Codonopsis Radix and for the determination of lobetyolin. Methods HPLC coupled with diode array detection method was employed to establish the fingerprint profile and quantitative determination of lobetyolin in Codonopsis Radix. Principal component analysis method was employed to analyze the 52 Codonopsis Radix samples. Results The reference chromatogram was generated with 25 common peaks showing good separation from adjacent peaks. Conclusion Statistical analysis of the obtained data demonstrates that the developed HPLC fingerprint combined with chemometric is a reliable method for the similar evaluation and quality assessment of Codonopsis Radix, and other traditional Chinese herbs.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::6f3e3f0a923497c69e6ec4e19ee5f9c4 https://doi.org/10.1016/s1674-6384(13)60046-5 Zobrazit plný text záznamu Full Text from ScienceDirect