Octreotide regulates CC but not CXC LPS-induced chemokine secretion in rat Kupffer cells
| Autor: | George Kolios, Elias A. Kouroumalis, C. Xidakis, Vassilis Valatas, George Notas, Pinelopi Manousou, Ioannis Diamantis |
|---|---|
| Rok vydání: | 2004 |
| Předmět: |
Pharmacology
Liver injury medicine.medical_specialty Chemokine biology Lipopolysaccharide Octreotide medicine.disease chemistry.chemical_compound Endocrinology Somatostatin Downregulation and upregulation chemistry Internal medicine Chemokine secretion medicine biology.protein Secretion medicine.drug |
| Zdroj: | British Journal of Pharmacology. 141:477-487 |
| ISSN: | 0007-1188 |
| DOI: | 10.1038/sj.bjp.0705633 |
| Popis: | Kupffer cells (KC) and lipopolysaccharide (LPS) interaction is the initial event leading to hepatic inflammation and fibrosis in many types of liver injury. We studied chemokine secretion by KC activated with LPS and the possible effect of the somatostatin analogue octreotide, in the regulation of this process. KC isolated from Sprague-Dawley rats were cultured in the presence of LPS added alone or with different concentrations of octreotide for 24 and 48 h, and chemokine production was assessed in culture supernatants by ELISA. CC chemokine mRNA expression was assessed by semiquantitative RT-PCR. Vehicle-stimulated KC produced a basal amount of CC and CXC chemokines. LPS-stimulated KC secreted significantly increased amounts of IL-8 (GRO/CINC-1) (P |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|