Tamarix gallica phenolics protect IEC-6 cells against H2O2 induced stress by restricting oxidative injuries and MAPKs signaling pathways
| Autor: | Marie-Agnès Giroux-Metges, Mondher Boulaaba, Christian Magné, Hélène Talarmin, Jamila Bettaib, Mickaël Droguet, Riadh Ksouri |
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| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Pharmacology Programmed cell death Antioxidant ABTS biology Chemistry medicine.medical_treatment General Medicine Oxidative phosphorylation medicine.disease_cause biology.organism_classification Cytoprotection Tamarix gallica 03 medical and health sciences chemistry.chemical_compound 030104 developmental biology 0302 clinical medicine Biochemistry 030220 oncology & carcinogenesis medicine Viability assay Oxidative stress |
| Zdroj: | Biomedicine & Pharmacotherapy. 89:490-498 |
| ISSN: | 0753-3322 |
| Popis: | Polyphenolic compounds gained interest in the pharmaceutical research area due to their beneficial properties. Herein, antioxidant and cytoprotective capacities of T. gallica extract on H2O2-challenged rat small intestine epithelial cells were investigated. To set stress conditions, IEC-6 cultures were challenged with numerous H2O2 doses and durations. Then, 40μM H2O2 during 4h were selected to assess the cytoprotective effect of different T. gallica extract concentrations. Oxidative parameters, measured through CAT and SOD activities as well as MDA quantification were assessed. In addition, the expression of possibly involved MAPKs was also valued. Main results reported that T. gallica was rich in polyphenols and exhibited an important antioxidant activity (DPPH Assay, IC50=6μgmL-1; ABTS+ test, IC50=50μgmL-1; Fe-reducing power, EC50=100μgmL-1). The exposure of IEC-6 cultures to 40μM H2O2 during 4h caused oxidative stress manifested by (i) over 70% cell mortality, (ii) over-activity of CAT (246%), (iii) excess in MDA content (18.4nmolmg-1) and (iiii) a trigger of JNK phosphorylation. Pretreatment with T. gallica extract, especially when used at 0.25μgmL-1, restored cell viability to 122%, and normal cell morphology in H2O2-chalenged cells. In addition, this extract normalized CAT activity and MDA content (100% and 14.7nmolmg-1, respectively) to their basal levels as compared to control cells. Furthermore, stopping cell death seems to be due to dephosphorylated JNK MAPK exerted by T. gallica bioactive compounds. In all, T. gallica components provided a cross-talk between regulatory pathways leading to an efficient cytoprotection against harmful oxidative stimulus. |
| Databáze: | OpenAIRE |
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