Caspofungin-inducedin-vitropost-antifungal effect and its impact on adhesion related traits of oralCandida dubliniensisandCandida albicansisolates
Autor: | Zia U. Khan, Arjuna N.B. Ellepola, Lakshman P. Samaranayake, Rachel Chandy |
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Rok vydání: | 2016 |
Předmět: |
0301 basic medicine
biology 030106 microbiology Immunology Virulence Germ tube bacterial infections and mycoses biology.organism_classification Microbiology In vitro 03 medical and health sciences Minimum inhibitory concentration chemistry.chemical_compound 030104 developmental biology chemistry Virology polycyclic compounds Caspofungin skin and connective tissue diseases Cell adhesion Candida albicans Candida dubliniensis |
Zdroj: | Microbiology and Immunology. 60:160-167 |
ISSN: | 0385-5600 |
Popis: | Adhesion to buccal epithelial cells (BEC) and denture acrylic surfaces (DAS), germ tube (GT) formation and cell surface hydrophobicity (CSH) are all virulence traits involved in the pathogenicity of Candida. Post-antifungal effect (PAFE) also have a bearing on pathogenicity and virulence of Candida. Candida dubliniensis is associated with oral and systemic candidosis, which can be managed with caspofungin. There is no published information on caspofungin-induced PAFE and its impact on adhesion traits of C. dubliniensis isolates. Thus, the purpose of this investigation was to determine the in vitro duration of PAFE on 20 C. dubliniensis isolates following transient exposure to caspofungin. Furthermore the impacts of caspofungin-induced PAFE on adhesion to BEC and DAS, GT formation and CSH of these isolates were also determined. After establishing the minimum inhibitory concentration (MIC) of caspofungin, C. dubliniensis isolates were exposed to sub-lethal concentrations (×3 MIC) of caspofungin for 1 hr. Thereafter the duration of PAFE, adhesion to BEC and DAS, GT formation and CSH were determined by previously described in-vitro assays. MIC (μg/mL) of C. dubliniensis isolates to caspofungin ranged from 0.004 to 0.19. Caspofungin-induced mean PAFE on C. dubliniensis isolates was 2.17 hr. Exposure to caspofungin suppressed the ability of C. dubliniensis isolates to adhere to BEC and DAS, form GT and CSH by 69.97%, 71.95%, 90.06% and 32.29% (P |
Databáze: | OpenAIRE |
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