Erythropoiesis in vitro: enhancement by neuraminidase

Autor:	Barbara A. Farley, Peter T. Rowley, Betsy M. Ohlsson-Wilhelm
Rok vydání:	1981
Předmět:	Fetus biology Immunology Stimulation Cell Biology Hematology Biochemistry Molecular biology In vitro Fetal cell Erythropoietin hemic and lymphatic diseases Human fetal biology.protein medicine Erythropoiesis Neuraminidase medicine.drug
Zdroj:	Blood. 57:483-490
ISSN:	1528-0020 0006-4971
DOI:	10.1182/blood.v57.3.483.483
Popis:	Neuraminidase treatment of human fetal liver or adult marrow cells prior to culture results in an increased number of erythroid colonies and bursts. No increase occurs in the number of nonerythroid colonies. The number of bursts having more than eight subunits is increased preferentially. Individual burst subunits are also enlarged. Neuraminidase-treated cells yield erythroid bursts when cultured in concentrations of erythropoietin insufficient to produce bursts from untreated cells. It is proposed that (1) neuraminidase treatment of adult and fetal cell mixtures specifically stimulates differentiation of erythroid precursors, (2) the preferential stimulation of erythroid bursts having many subunits suggests a preferential susceptibility of more primitive BFU-Es, and (3) neuraminidase treatment enhances the response of erythroid precursors to erythropoietin.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::6dafe4894d189f6ad4882e0801576bd8 https://doi.org/10.1182/blood.v57.3.483.483 Zobrazit plný text záznamu