Epitope mapping of prostate-specific antigen with monoclonal antibodies

Autor:	F.T. Kreutz, David S. Wishart, Mavanur R. Suresh, Antoine A. Noujaim, D.C. Jette, Bruce A. Malcolm
Rok vydání:	1996
Předmět:	Linear epitope medicine.drug_class Biochemistry (medical) Clinical Biochemistry Biology urologic and male genital diseases Monoclonal antibody Molecular biology Epitope Prostate-specific antigen Epitope mapping Antigen Immunology medicine biology.protein Antibody Peptide library
Zdroj:	Clinical Chemistry. 42:1961-1969
ISSN:	1530-8561 0009-9147
DOI:	10.1093/clinchem/42.12.1961
Popis:	Prostate-specific antigen (PSA) is a widely used marker for screening and monitoring prostate cancer. We identified and characterized the epitopes of two anti-PSA monoclonal antibodies (mAbs) designated B80 and B87. The epitopes were initially mapped as nonoverlapping by developing a sandwich immunoassay to measure PSA with the two anti-PSA mAbs. The two antibodies do not cross-react with homologous pancreatic kallikrein, but recognize epitopes unique to PSA. B80 and B87 can recognize both free and complexed PSA and hence measure total PSA. Epitope scanning and bacteriophage peptide library affinity selection procedures were used to identify and locate an epitope on PSA. A possible epitope for B80 was identified as being located on or near PSA amino acid residues 50-58 (-GRH-SLFHP-). The epitope for B87 was likely on an exposed nonlinear conformational determinant, unique to PSA, and not masked by the binding of B80 or alpha 1-antichymotrypsin.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::6d9731b64764454c451337daffddc657 https://doi.org/10.1093/clinchem/42.12.1961 Zobrazit plný text záznamu