| Předmět: |
0301 basic medicine
Pharmacology chemistry.chemical_classification Pombalia calceolaria 010405 organic chemistry Organic Chemistry Pharmaceutical Science Cell migration 01 natural sciences Molecular biology 0104 chemical sciences Analytical Chemistry Amino acid 03 medical and health sciences 030104 developmental biology Complementary and alternative medicine chemistry Cyclotides Drug Discovery Cancer cell Molecular Medicine Cytotoxic T cell Breast cancer cells Cytotoxicity |
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| Zdroj: | Journal of Natural Products. |
| ISSN: | 0163-3864 |
| Popis: | Two new bracelet cyclotides from roots of Pombalia calceolaria with potential anticancer activity have been characterized in this work. The cyclotides Poca A and B (1 and 2) and the previously known CyO4 (3) were de novo sequenced by MALDI-TOF/TOF mass spectrometry (MS). The MS2 spectra were examined and the amino acid sequences were determined. The purified peptides were tested for their cytotoxicity and effects on cell migration of MDA-MB-231, a triple-negative breast cancer cell line. The isolated cyclotides reduced the number of cancer cells by more than 80% at 20 μM, and the concentration-related cytotoxic responses were observed with IC50 values of 1.8, 2.7, and 9.8 μM for Poca A (1), Poca B (2), and CyO4 (3), respectively. Additionally, the inhibition of cell migration (wound-healing assay) exhibited that CyO4 (3) presents an interesting activity profile, in being able to inhibit cell migration (50%) at a subtoxic concentration (2 μM). The distribution of these cyclotides in the roots was analyzed ... |
| Databáze: | OpenAIRE |
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