Influence of tetrapeptide tuftsin on intracellular pH of mouse peritoneal macrophages
| Autor: | V. B. Turovetsky, Pirutin Sk, V. N. Kalihevich, N. Y. Sarycheva, A. A. Kamensky, Anna B. Druzhko |
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| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Tetrapeptide Chemistry Intracellular pH Tuftsin Phi value analysis Microfluorimetry General Biochemistry Genetics and Molecular Biology 03 medical and health sciences Sodium–hydrogen antiporter chemistry.chemical_compound 030104 developmental biology 0302 clinical medicine Biochemistry Biophysics Cell response General Agricultural and Biological Sciences Incubation 030217 neurology & neurosurgery General Environmental Science |
| Zdroj: | Moscow University Biological Sciences Bulletin. 71:58-61 |
| ISSN: | 1934-791X 0096-3925 |
| Popis: | It has been shown by the method of probe microfluorimetry of single cells that tuftsin at concentrations of 0.1 and 1.0 μg/mL, increasing the phagocytic activity of murine peritoneal macrophages, causes biphasic changes in the intracellular pH (pHi) over time. First there was a decrease in pHi, reaching the limit in 5 min of incubation. Then, the pHi value increased until reaching the maximum 30 min after the interaction between the cells and the agent. Afterwards, the observed parameter (pHi) did not vary up to minute 55. It was shown that there was no increase in intracellular pH on the addition of the Na+/H+-exchange blocker ethylisopropylamiloride to the incubation medium in the presence of tuftsin. This fact suggests that the observed increase in pHi during tuftsin treatment in the second phase of cell response is associated with the Na+/H+ exchange system. |
| Databáze: | OpenAIRE |
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