Effects of Propylthiouracil on the Production of Aldosterone in Rat Zona Glomerulosa Cells
| Autor: | Paulus S. Wang, Chia-Wen Lin, Hsiao-Fung Pu, Hsiao-Wei Lee |
|---|---|
| Rok vydání: | 2012 |
| Předmět: |
Aldosterone synthase
endocrine system medicine.medical_specialty Aldosterone Forskolin biology Chemistry Adrenal gland Cholesterol side-chain cleavage enzyme General Medicine Angiotensin II chemistry.chemical_compound medicine.anatomical_structure Endocrinology Zona glomerulosa Corticosterone Internal medicine medicine biology.protein hormones hormone substitutes and hormone antagonists |
| Zdroj: | Adaptive Medicine. 4:245-250 |
| ISSN: | 2076-944X |
| DOI: | 10.4247/am.2012.abc045 |
| Popis: | Propylthiouracil (PTU), an anti-thyroid drug, is widely used for the treatment in hyperthyroid patients. Previous studies indicate that thyroxine inhibit the spontaneous and adrenocorticotropin (ACTH)-stimulated corticosterone secretion by acting directly at adrenal glands via decrease cAMP production. It has also been shown that PTU suppresses corticosterone release by male rat zona fasciculata-reticularis cells. However, the direct effects and mechanisms of PTU on the aldosterone production in adrenal zona glomerulosa (ZG) cells are still unclear. ZG cells were prepared from adrenocortical tissues of male rats, and then challenged with or without angiotensin II (10^(-7), 10^(-6) M), ACTH (10^(-9) M), A23187 (10^(-5) M), cyclopiazonic acid (CPA, 10^(-5) M), forskolin (10^(-5) M), 8-Br-cAMP (10^(-5) M), trilostane (10^(-6) M, 3β-HSD inhibitor) or steroidogenic precursors (e.g. 10^(-6) M 25-OH-cholesterol and 10^(-7), 10^(-5) M corticosterone) at 37°C for 1 h. Our results showed that PTU (0-3 mM) dose-dependently decreased the aldosterone release in response to the above hormones, drugs and the steroidogenic precursors in vitro. PTU also decreased steroidogenic acute regulatory (StAR) protein expression, but did not alter the protein expression of P450 side chain cleavage enzyme (P450scc) during steroidogenesis of aldosterone. These results suggested that PTU has a direct inhibitory effect on aldosterone production via cAMP, and Ca^2+ down stream pathway and the steroidogenic enzymes activities including P450scc and aldosterone synthase, as well as StAR protein expression. |
| Databáze: | OpenAIRE |
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