Isolation and characterization of 14 microsatellite markers for Rhizophora mucronata (Rhizophoraceae) and their potential use in range-wide population studies

Autor: Sankararamasubramanian Halasya Meenakshisundaram, Emiko Oguri, Orlex B. Yllano, M. Nazre Saleh, Bayu Adjie, Tadashi Kajita, Norhaslinda Binti Malekal, Severino G. Salmo, Noriaki Murakami, Edward L. Webb, Takeshi Asakawa, Erwin Riyanto Ardli, Yasuyuki Watano, Onrizal, Nguyen Xuan Tung, Sarawood Sungkaew, Alison K. S. Wee, Shigeyuki Baba, Koji Takayama, Yoshimi Shinmura, Khin Khin Soe
Rok vydání: 2012
Předmět:
Zdroj: Conservation Genetics Resources. 4:951-954
ISSN: 1877-7260
1877-7252
DOI: 10.1007/s12686-012-9681-y
Popis: A set of 14 new microsatellite markers was developed for mangrove species Rhizophora mucronata (Rhizophoraceae) by using pyrosequencing. Fifty-six samples from 9 populations of R. mucronata in the Indo-West Pacific region were genotyped; all loci were polymorphic, with the number of alleles ranging from 2 to 9. The mean expected heterozygosity per locus was 0.16 in a population from Sabah, no significant linkage disequilibrium was found among loci, and significant deviation from Hardy–Weinberg equilibrium was found in 3 loci. The polymorphic microsatellite markers with samples covering most of the species’ distribution range can be applied in genetic diversity studies covering a broad geographical range of the species.
Databáze: OpenAIRE