Studies on Ricinus Lipase Enzyme Isolated from Castor Seeds
| Autor: | Shashi Agrawal, Alka Srivastava, Rachna Prakash, Rashmi Mohan Mathur |
|---|---|
| Rok vydání: | 2015 |
| Předmět: |
Phytochemistry
02 engineering and technology 010402 general chemistry 01 natural sciences Biochemistry law.invention Sieve law Drug Discovery Environmental Chemistry Organic chemistry Lipase Chromatography biology Glass Vial Chemistry Soxhlet extractor Extraction (chemistry) Ricinus General Chemistry 021001 nanoscience & nanotechnology biology.organism_classification 0104 chemical sciences biology.protein Desiccator 0210 nano-technology |
| Zdroj: | Oriental Journal of Chemistry. 32:1235-1247 |
| ISSN: | 2231-5039 0970-020X |
| DOI: | 10.13005/ojc/320250 |
| Popis: | Healthy and bold seeds of castor variety "Aruna" were collected. The de-hulled castor kernels were separated from seed coat and finely grounded in an electric grinder. The powdered kernels were passed through 60 mesh sieve and defatted using n-hexane in a soxhlet extractor. After complete extraction of oil seed meal was made free from solvent at low temperature. The defatted seed meal was pulverized and again passed through 60-mesh sieve to get final product .The RICINUS LIPASE source thus, obtained was stored in a screw capped glass vial and kept in a desiccator. As and when required portions from this preparation were used in further enzymatic studies. |
| Databáze: | OpenAIRE |
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