The prevention of saltwater algal pond contamination using the electron transport chain disruptor, rotenone

Autor: Steven W. Van Ginkel, Ruben Gijon-Felix, Zixuan Hu, Luis Henrique Kalil Duarte, Micah Bidwell, Yongsheng Chen, Djessica Steiner, Thomas Igou, Silvia Helena Rego De Oliveira, Emille Jeane Novais Ribeiro Salvi, Rachel K. Johnston, Terry W. Snell
Rok vydání: 2016
Předmět:
Zdroj: Algal Research. 18:209-212
ISSN: 2211-9264
DOI: 10.1016/j.algal.2016.06.012
Popis: Rotenone is a classic inhibitor of Complex I of the electron transport chain which is common to all life. Algae are known to be rotenone-insensitive because in addition to Complex I they have alternative pathways for the oxidation of NADH 2 . It is this differential sensitivity to rotenone that can be exploited to reduce many types of predators while not affecting biodiesel-producing algae. A universal tool like rotenone is needed to reduce predation in open ponds as predation is well known to limit cultivation to a few weeks in the warmer months. In this study, we tested the effect of rotenone on the mortality and reproduction of two marine algae strains, Nannochloropsis oculata and Tetraselmis suecica , and two marine rotifer strains, Brachionus manjavacas and Brachionus rotundiformis . The LC 50 s for the two rotifers ranged from 0.18 to 0.35 μM while N . oculata was unaffected to at least 7.6 μM. Rotifer reproduction was reduced at rotenone concentrations lower than those that caused mortality. Rotenone applications to produce 1 million gallons of biodiesel in one year are estimated to cost $1684. This provides algae farmers an important and inexpensive tool to manage grazing predators in algae mass cultures and avoid pond crashes.
Databáze: OpenAIRE
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