Effect of Triton X-100 on Tegument and Muscle in Schistosoma mansoni
| Autor: | James L. Bennett, Connie S. Bricker, Joseph W. Depenbusch, Ralph A. Pax |
|---|---|
| Rok vydání: | 1982 |
| Předmět: |
Membrane potential
Voltage-dependent calcium channel biology Depolarization Stimulation biology.organism_classification chemistry.chemical_compound Biochemistry chemistry Muscle tension Triton X-100 Biophysics medicine Parasitology Schistosoma mansoni medicine.symptom Ecology Evolution Behavior and Systematics Muscle contraction |
| Zdroj: | The Journal of Parasitology. 68:884 |
| ISSN: | 0022-3395 |
| DOI: | 10.2307/3281000 |
| Popis: | Short periods of in vitro incubation of Schistosoma mansoni with low concentrations of the nonionic detergent Triton X-100 caused marked changes in the physiological and morphological status of the parasite. Less than 15 min of exposure to 1 mM Triton at 37 C led to irreversible destruction of the tegument, relaxation and depolarization of the muscle, and loss of response to electrical stimulation, to 60 mM K+-HBS, or to 10(-6) M praziquantel. A 5-min incubation in 100 microM Triton X-100 caused no gross structural damage, changes in tegumental or E2 electrical potential, or decrease in response to praziquantel, but markedly inhibited the ability of 60 mM K+-HBS or electrical stimulation to cause muscle contraction. Radioactive calcium influx measurements indicated that a 5-min exposure to 100 microM Triton X-100 may be uncoupling membrane depolarization from development of muscle tension by interfering pharmacologically with calcium channels. Longer incubations (15 min) in the presence of 100 microM Triton X-100 caused transitional physiological changes suggesting that the mode of action of the detergent was passing from a specific, pharmacological mode to a nonspecific disruptive action. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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